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Crystal violet

Crystal violet

Crystal violet


Crystal violet or gentian violet (also known as methyl violet 10B or hexamethyl pararosaniline chloride) is a triarylmethane dye used as a histological stain and in Gram's method of classifying bacteria. Crystal violet has antibacterial, antifungal, and anthelmintic properties and was formerly important as a topical antiseptic. The medical use of the dye has been largely superseded by more modern drugs, although it is still listed by the World Health Organization.
The name "gentian violet" was originally used for a mixture of methyl pararosaniline dyes (methyl violet), but is now often considered a synonym for crystal violet. The name refers to its colour, being like that of the petals of a gentian flower; it is not made from gentians or from violets.

Grade:AR Grade

Purity:99.9% min

CAS Number : 548-62-9

EC Number :208-953-6

Molecular Formula:C25H30N3Cl

Technical Requirements:


Crystal violet

Appearance Dark-green Crystal Powder
 Ignition Residue (w/%) 0.5% max
 Loss on Drying (w/%) 6.0 % max
Solubility in Ethanol Qualified
Absorption Test Qualified


Crystal violet is not used as a textile dye. Instead, it is used to dye paper and as a component of navy blue and black inks for printing, ball-point pens, and ink-jet printers. It is also used to colourize diverse products such as fertilizers, antifreezes, detergents, and leather.
The dye is also used as a histological stain, particularly in Gram staining for classifying bacteria.
When conducting DNA gel electrophoresis, crystal violet can be used as a nontoxic DNA stain as an alternative to fluorescent, intercalating dyes such as ethidium bromide. Used in this manner, it may be either incorporated into the agarose gel or applied after the electrophoresis process is finished. Used at a 0.001% concentration and allowed to stain a gel after electrophoresis for 30 minutes, it can detect as little as 16ng of DNA. Through use of a methyl orange counterstain and a more complex staining method, sensitivity can be improved further to 8 ng of DNA. When crystal violet is used as an alternative to fluorescent stains, it is not necessary to use ultraviolet illumination; this has made crystal violet popular as a means of avoiding UV-induced DNA destruction when performing DNA cloning in vitro.
In forensics, gentian violet was used to develop fingerprints. Crystal violet is also used as a tissue stain in the preparation of light microscopy sections. In laboratory, solutions containing crystal violet and formalin are often used to simultaneously fix and stain cells grown in tissue culture to preserve them and make them easily visible, since most cells are colourless. It is also sometimes used as a cheap way to put identification markings on laboratory mice; since many strains of lab mice are albino, the purple colour stays on their fur for several weeks.
In body piercing, gentian violet is commonly used to mark the location for placing piercings, including surface piercings.
Crystal Violet is the primary stain used in the Gram stain technique for differentiation of Gram-negative versus Gram-postive bacteria.
Used as a component of CV cytotoxicity assays.

Packing:25g/bottle or according to your requirement.

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